Device

Part:BBa_K2150110

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-13)
Revision as of 08:37, 13 October 2016 by Zepeng Mu (Talk | contribs)


LacI (constitutive) + Ptac + RiboJ + 1204 toxin

This composite part consists of LacI under constitutive promoter, and toxin gene of toxin N-acetyltransferase GCN5 (In our project, ID: 1204) under pTac prompter. Directly upstream of coding region of toxin gene is RiboJ, an insulator buffers gene expression from its cellular context. RIboJ is a ribozyme which effetely cleaves 5'-UTR of mRNAs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1611
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1857
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1611
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1611
    Illegal NgoMIV site found at 1788
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
Parameters
None