Deleted
Sample Discontinued
Experience: None
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Part:BBa_K2150021

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 04:17, 13 October 2016 by Freedom.H@UCAS (Talk | contribs)

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pCon+RBS+tetR+DT+pT7+pTet+RBS+GFP+RBS+T7RNAP+DT

This part is a complete regulatory device sensitive to tetracycline. The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1643


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