Composite

Part:BBa_K1900000:Design

Designed by: Elise Sloey   Group: iGEM16_WLC-Milwaukee   (2016-10-12)
Revision as of 16:51, 20 October 2016 by Elisesloey (Talk | contribs) (→‎Design Notes)


pBAD+strong RBS+E. coli tolC signal sequence+K. pneumoniae tolC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1603
    Illegal NheI site found at 1624
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1104


Design Notes

BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Klebsiella pneumoniae TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be BioBrick compatible.

Source

This part

References