Regulatory

Part:BBa_K1980006:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 22:59, 23 October 2016 by Sgarforth (Talk | contribs) (→‎Source)


pCopA with divergent expressed CueR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 438
    Illegal NheI site found at 461
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed so that any protein can be ligated to this part with standard assembly to make them respond to copper (for instance our copper chelator proteins)

Design Notes

Codon optimised. Designed for biobrick assembly with protein/reporter of choice.


Source

The promoter originates from E. coli. Ordered as codon optimised DNA from IDT.

References