Coding
MymT

Part:BBa_K1980002:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 21:09, 23 October 2016 by Sgarforth (Talk | contribs) (→‎References)

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MymT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.


Source

MymT is originally from Mycobacterium tuberculosis. We ordered it as codon optimised DNA from IDT.

References

Ben Gold, Haiteng Deng, Ruslana Bryk, Diana Vargas, David Eliezer, Julia Roberts, Xiuju Jiang, & Carl Nathan (2009) “Identification of a Copper-Binding Metallothionein in Pathogenic Mycobacteria” Nat Chem Biol. 2008 October ; 4(10): 609–616. doi:10.1038/nchembio.109.