Part:BBa_K1884009:Design

PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3123
  Illegal NotI site found at 3221
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1560
  Illegal BglII site found at 2019
  Illegal BamHI site found at 2498
  Illegal XhoI site found at 3052
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1444
  Illegal AgeI site found at 2173
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1796
  Illegal BsaI.rc site found at 1205
  Illegal SapI.rc site found at 1313
  Illegal SapI.rc site found at 3384
  

References

[1]M. Winkler, A. Hemschemeier, J. Jacobs, S. Stripp, T. Happe Multiple ferredoxin isoforms in Chlamydomonas reinhardtii-their role under stress conditions and biotechnological implications Eur J Cell Biol, 89 (2010), pp. 998–1004 [2]I. Yacoby, S. Pochekailov, H. Toporik, M.L. Ghirardi, P.W. King, S.G. Zhang Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin: NADP+-oxidoreductase (FNR) enzymes in vitro Proc Natl Acad Sci USA, 108 (2011), pp. 9396–9401 [3]M. Chen, L. Zhao, Y.L. Sun, S.X. Cui, L.F. Zhang, B. Yang, et al. Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells J Proteome Res, 9 (2010), pp. 3854–3866