Regulatory

Part:BBa_I760005

Designed by: Gennady Zakharov   Group: iGEM07_Saint_Petersburg   (2007-08-20)
Revision as of 17:26, 11 October 2016 by Sgarforth (Talk | contribs)

Cu-sensitive promoter

Promoter from the copper-sensitive CusR/CusS two component signal system. (E.Coli)

Usage and Biology

This nucleotide sequence is believed to be able to bind with phosphorylated CusR transcription factor in E.coli. CusR protein is phosphorylated by CusS transmembrane protein in a case of high extracellular concentration of copper ions. After phosphorylation CusR interacts with described DNA sequence and activates the transcription of CusA, CusB, CusC and CusF genes coding the proteins of copper metabolic system. Was used by Saint-Petersburg Team for constructing a copper biosensor system.

This part was updated by Oxford iGEM in 2016 by adding on an long upstream sequence. The resulting part was functional. <a href="https://parts.igem.org/Part:BBa_K1980000">here.</a>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/sensor/metal
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
negative_regulators
positive_regulators1