Composite
Part:BBa_K1897028
Designed by: Keshiniy Madivannan Group: iGEM16_NUS_Singapore (2016-10-11)
p70-33-sfGFP-Terminator
The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (Part: BBa_K1847008) with a weaker RBS flanked by non-coding sequences (Part: BBa_K1897031), followed by a CDS of sfGFP (Part: BBa_K1897033) and a lambda t0 terminator (Part: BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 589
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |