Intermediate
T2A

Part:BBa_K1993019

Designed by: Su Xiaojun   Group: iGEM16_SYSU-MEDICINE   (2016-10-11)
Revision as of 07:40, 16 October 2016 by Icyvodka (Talk | contribs)


T2A

T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. It is a good candidate to replace IRES because of its small size and high cleavage efficiency between genes upstream and downstream of the 2A peptide. [1]

In our project, we found that protein coding genes downstream were not expressed if they followed another protein coding gene directly. Taking advantage of function of T2A, we constructed a plasmid that T2A linked between two protein coding genes. For example, we constructed BBa_K1993006 (Luciferase-T2A-dTomato-T2A-hFTH) to ensure luciferase, dTomato and hFTH could be expressed.

References

[1] Kim J H, Lee S R, Li L H, et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. [J]. Plos One, 2011, 6(4): e18556.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None