Composite

Part:BBa_K1926011

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 08:58, 11 October 2016 by CindyLiu (Talk | contribs)

The SNAP UNIT: SNAP-tag flanked by loxP

This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

You may use this part to:

1) Localize proteins in vivo with BLOCK and dye from NEB company;

2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus.


Source

The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.


Construction Design

[edit]
Categories
//chassis/eukaryote/human
//function/recombination/cre
Parameters
None