Coding

Part:BBa_K2114019:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-10)
Revision as of 06:37, 20 October 2016 by D.Steinberg (Talk | contribs)

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GST_HA_G4S_CgeA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1008
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.


Source

The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

References