Coding

Part:BBa_K1897002:Design

Designed by: Choi Yan Ru   Group: iGEM16_NUS_Singapore   (2016-10-09)
Revision as of 18:49, 10 October 2016 by Cyr95 (Talk | contribs) (→‎References)

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HasR Membrane receptor coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1866
    Illegal NotI site found at 2660
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 24
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 84
    Illegal NgoMIV site found at 202
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 421
    Illegal NgoMIV site found at 757
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NIL


Source

The HasR gene is originally found in the Serratia marcescens. We synthesised the part from the Serratia marcescens genome, obtaining the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2).

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Rossi, M. S., Paquelin, A., Ghigo, J. M., & Wandersman, C. (2003). Haemophore‐mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules. Molecular microbiology, 48(6), 1467-1480.