Part:BBa_K1897002:Design

HasR Membrane receptor coding sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1866
  Illegal NotI site found at 2660
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 24
  Illegal NgoMIV site found at 28
  Illegal NgoMIV site found at 84
  Illegal NgoMIV site found at 202
  Illegal NgoMIV site found at 356
  Illegal NgoMIV site found at 421
  Illegal NgoMIV site found at 757
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

NIL

Source

The HasR gene is originally found in the Serratia marcescens. We synthesised the part from the Serratia marcescens genome, obtaining the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2).

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Rossi, M. S., Paquelin, A., Ghigo, J. M., & Wandersman, C. (2003). Haemophore‐mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules. Molecular microbiology, 48(6), 1467-1480.