Scar

Part:BBa_K2042019:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-09-11)
Revision as of 20:54, 12 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)

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Spacer in between promoter and coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was designed as a spacer to prevent errors in transcription by placing the RBS close to the promoter. Formation of a hairpin structure or self complementary sequences was avoided.

Source

Synthetic synthesis

References