DNA

Part:BBa_K2100010:Experience

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)
Revision as of 00:00, 18 October 2016 by Sarahcaso (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2100010

We characterized our hybrid promoter in both MCF-7 and tHESC cells. The following characterization experiment in tHESC cells tested how the hybrid promoter responds to a 1 uM induction of progesterone (MPA). The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.

http://2016.igem.org/File:T--MIT--hybridthesc.png

The next characterization experiment tested how the hybrid promoter responds to a 10 nM induction of E2 (estradiol) in MCF-7 cells. The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.

http://2016.igem.org/File:T--MIT--hybridmcf7.png

The results showed an 100 fold difference between the yellow fluorescent output of the uninduced tHESC cells and the ones induced with 1 uM MPA.


User Reviews

UNIQb0ecea3f9ec2cceb-partinfo-00000000-QINU UNIQb0ecea3f9ec2cceb-partinfo-00000001-QINU