Part:BBa_K1898300

CRYAA, crystallin alpha A

This gene codes for CRYAA, or crystallin alpha A, a crystallin protein that is more restricted to the lens.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 59
  
• 1000
  COMPATIBLE WITH RFC[1000]

Gel Pictures

We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. As shown below, the bands with the expected size are boxed in red:

Sequencing

CRYAA DNA was sent to sequencing after it's moved into an iGEM backbone. The sequencing result confirms that CRYAA is right.

• The four enzyme cutting sites are highlighted in red
• CRYAA is highlighted in light blue