DNA

Part:BBa_K2100007

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)
Revision as of 23:20, 17 October 2016 by Sarahcaso (Talk | contribs)


pENTR tagBFP

tagBFP is a monomeric blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter.[1] It is the brightest of the blue fluorescent proteins. We used hEF1a-tagBFP as a transfection marker in several of our experiments.

[1] http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0028674


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None