Part:BBa_K1898200

GSR, glutathione reductase

GSR codes for gluatathione reductase, an enzyme that catalyzes the conversion of glutathione reductase to glutathione. Glutathione is an antioxidant exists in humans, plants, fungi, animal that prevents cellular damage by reactive oxygen species. [1] In our project, we aim to deliver GSR to the lens, where GSH will be produced and prevent reactive oxygen species from damaging the lens proteins. Age-induced cataract is mainly caused by reactive oxygen species oxidation of the lens protein. The increase in GSH will help prevent cataracts from happening.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 743
  Illegal BamHI site found at 1427
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 28
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1138
  Illegal SapI.rc site found at 1546
  

Gel Pictures

We designed primers synthesized by Tri-I Biotech to remove stop codon and to move GSR cDNA to iGEM BioBrick. PCR was set up and the gel picture is shown above. The expected size for GSR PCR check is ~2kb, which is shown in lane__:

Sequencing

The sequencing result shows that the GSR sequencing result is correct (with no stop codon):