Regulatory

Part:BBa_K1679042

Designed by: Qikai Qin   Group: iGEM15_OUC-China   (2016-10-10)
Revision as of 11:55, 10 October 2016 by QinQikai (Talk | contribs)


RiboJ

After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin immediately downstream to help expose the RBS. STRSV can cleaves the mRNA autocatalytically at a defined residue, which had the effect of cleaving extraneous RNA leaders that arose from transcription from promoters with different start sites.[1] Attributed to the biochemical function mentioned above, RiboJ has insulating capability. We use it to buffer synthetic circuits from genetic context.

Attention Please:

The DNA sample is the same as BBa_K1679038. There is a mistake of sequence information in BBa_K1679038, but the DNA sample is correct. The sequence information is right here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None