Device

Part:BBa_K2088672

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-09)
Revision as of 15:58, 9 October 2016 by Summerice (Talk | contribs)


copA + riboJ +mhbDHIM + terminator

This composite part uses a promoter induced by copper ions(BBa_K2088001）， riboJ（BBa_K2066506）， mhbD with native rbs（BBa_K2088667）, mhbH with native rbs(BBa_K2088670), mhbI with native rbs(BBa_K2088669), mhbM with native rbs(BBa_K2088668), and a double terminator(BBa_B0015). This part is used to degrade 3-hydroxybenzoate(3-HBA) to fumarate and pyruvate via the gentisate pathway induced by copper ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2518
    Illegal BamHI site found at 2904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal NgoMIV site found at 754
    Illegal NgoMIV site found at 2708
    Illegal NgoMIV site found at 3845
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3038


[edit]
Categories
Parameters
None