Generator

Part:BBa_K2070016

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-09)
Revision as of 01:25, 15 October 2016 by RinaSakata (Talk | contribs)


Constitutive ecf35 generator with ecf35 inducible GFP reporter

This part contains the ecf16 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf16 generated can activate the ecf16 promoter (BBa_K2070000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790
    Illegal BsaI.rc site found at 1543


[edit]
Categories
Parameters
None