Composite

Part:BBa_K2066031

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)
Revision as of 18:50, 12 October 2016 by Jlmaniaci (Talk | contribs)

pTAC cI-GFP

pTAC Ribozyme Characterization Part – cIGFP only; no RiboJ

This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_030 (pTAC Ribozyme Chracterization Part – GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 852


[edit]
Categories
Parameters
None