Composite
FimH-Dterm

Part:BBa_K2052014:Design

Designed by: Burak Kızıl, Seniz Yuksel   Group: iGEM16_METU_HS_Ankara   (2016-09-24)
Revision as of 01:18, 20 October 2016 by Maercyl (Talk | contribs) (→‎Source)


FimH site directed mutated with RPMrel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1173
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

....Enter any design considerations you had to deal with during the detailed design of the sequence.


Source

Some substrains of E.Coli have a structure called Type 1 pili which is expressed from the Fim gene system. At the end of the pili structure there is a protein called “FimH” which is the structure that allows them to bind to the mannose sugar that is found on the surfaces of eukaryotic cells. (Sauer et al., 2016)

References

Krogfelt KA., Bergmans H., Klemm P. (1990, June) “Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae”

Sauer MM, Jakob RP, Eras J, Baday S, Eriş D, Navarra G, Bernèche S, Ernst B, Maier T, Glockshuber R. (2016 March 7). “Catch-bond mechanism of the bacterial adhesin FimH”

Kelly, K. A., Jones, D. A., (2003). “Isolation of a Colon Tumor Specific Binding Peptide Using Phage Display Selection”

Figure 1. iGEM 2015 Harvard BioDesign . Retrieved from https://parts.igem.org/Part:BBa_K1850010

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1550331/


Induction protocol http://www.embl.de/pepcore/pepcore_services/protein_expression/ecoli/