Regulatory

Part:BBa_K2110007

Designed by: Le He   Group: iGEM16_Tianjin   (2016-10-06)
Revision as of 17:06, 13 October 2016 by Dhr9767 (Talk | contribs)

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TPA transporting protein gene expression system

This part consists of the enhanced promoter PGK1(BBa_K2110012), the tpaK gene(BBa_K2110003), and the terminator CYC1（BBa_K2110000）. The tpaK gene encodes the terephthalic acid (TPA) transporting protein. TPA is not a kind of nutrient substance for most organisms, including common chassis organisms like E.coli and Saccharomyces cerevisiae. However, as a kind of TPAdegradable organism, RhodococcusjostiiRHA1 can transport TPA into their cells because of the TPA transporting protein gene tpaK in their genome. This gene is also verified to be useful in Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1664
    Illegal BsaI.rc site found at 1951
    Illegal SapI site found at 1063
    Illegal SapI site found at 1330


[edit]
Categories
//regulation/positive
Parameters
chassissaccharomyces cerevisiae