Other
subE

Part:BBa_K2020000:Design

Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert   Group: iGEM16_Aachen   (2016-10-04)
Revision as of 20:07, 8 October 2016 by NicolaFreyer (Talk | contribs)


subtilisin E gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence from B. subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.


Source

The sequence was originally obtained from a wildtype Bacillus subtilis and was ordered from IDT.