Coding
pelB+subE

Part:BBa_K2020001:Design

Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert   Group: iGEM16_Aachen   (2016-10-04)
Revision as of 20:06, 8 October 2016 by NicolaFreyer (Talk | contribs)


subtilisin E gene with leader sequence pelB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000).
The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT. Moreover, the leader sequence pelB begins with a start codon and was placed in front of the subtilisin E gene to generate a functional coding sequence that can be expressed in E. coli.

Source

The sequence of the signal peptide pelB was obtained through the Registry (BBa_J32015). Together with the subtilisin E gene (BBa_K2020000) it was ordered from IDT.

References