Composite
ExpSubE
Part:BBa_K2020002
Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert Group: iGEM16_Aachen (2016-10-05)
Revision as of 17:03, 5 October 2016 by NicolaFreyer (Talk | contribs)
expression system for subtilisin E in E. coli
This composite part consists of the promoter BBa_R0010, the RBS BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for Escherichia coli codon usage (BBa_K2020000). Once introduced into E. coli, this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 280
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme/protease
//chassis/prokaryote/ecoli
//chassis/prokaryote/ecoli
biology | Bacillus subtilis |
chassis | Escherichia coli |
protein | subtilisin E |