Generator
Part:BBa_K1961004:Design
Designed by: Yi-Ting Lin Group: iGEM16_HSiTAIWAN (2016-10-03)
PbrR generator expressed by GFP under autoregulation of PTet
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2040
Design Notes
This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription.
Source
We use parts in the 2016 iGEM plate