Composite

Part:BBa_K2036024:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 09:44, 3 October 2016 by Shareycheng (Talk | contribs) (→‎Source)


placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3212
    Illegal BamHI site found at 3251
    Illegal BamHI site found at 3943
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a lactic acid balance circuit in E.coli with lactose signal to initiate. And when the pH environment are within 7 to 9,the host strain will prepare beta-galactosidase for next lactose input.
beta-galactosidase coding gene are alpha domain of laz,it functions only in E.coli strain which contains lacZ operon.

Source

iLDH are from the Registry and beta-galactosidase coding gene are referred to pUC119 commerial vector and synthesised by IDT.
CI,CII,CIII,Cro,pRE,pR,pRM are from bacteriaphage lambda.

References