Coding
tsPurple
Part:BBa_K1033906
Designed by: Sabri Jamal Group: iGEM13_Uppsala (2013-08-25)
Revision as of 08:29, 3 October 2016 by Saishreyas g (Talk | contribs)
tsPurple, purple chromoprotein
This chromoprotein (also known as TinselPurple) naturally exhibits strong purple color when expressed.
Usage and Biology
This part is useful as a reporter.
iGEM2013 Uppsala: The images above show E coli constitutively expressing the chromoproteins amilCP BBa_K592009 and tsPurple BBa_K1033906 from the high copy plasmid pSB1C3 from the promoters J23116 and J23110.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Structure and SWISS MODEL Homology Modelling Report
(The following information is the contribution of SVCE_Chennai)
The following sections give a detailed information on this chromoprotein done by its in silico analysis.
3D Structure
Cartoon representation of tsPurple.
QMEAN = 0.02
Cβ = -0.91
All Atom = 0.52
Solvation = -1.19
Torsion = 0.32
Template | Seq. Identity | Oligo-state | Found by | Method | Resolution | Seq Similarity | Range | Coverage | Description |
---|---|---|---|---|---|---|---|---|---|
4ohs.1.A | 86.61 | homotetramer | HHblits | X-Ray | 2.19Å | 0.58 | 2 - 222 | 0.98 | FAR-RED FLUORESCENT PROTEIN AQ143 |
Ligand | Added to Model | Description |
---|---|---|
CL | ✕ - Not biologically relevant. | CHLORIDE ION |
CL | ✕ - Not biologically relevant. | CHLORIDE ION |
CL | ✕ - Not biologically relevant. | CHLORIDE ION |
[edit]
Categories
Parameters
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
//collections/chromoprotein/uppsala
//function/reporter/pigment
color | Purple |