Regulatory

Part:BBa_K2092005:Design

Designed by: Hui Wen Chong   Group: iGEM16_Manchester   (2016-10-02)
Revision as of 22:42, 2 October 2016 by Huiwenchong (Talk | contribs) (→‎Design Notes)


alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2568
    Illegal BamHI site found at 1572
    Illegal BamHI site found at 2310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1276
    Illegal AgeI site found at 1404
    Illegal AgeI site found at 1935
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- Codon optimized the sequence with Thermo Fisher Scientific.

- Gene synthesized by IDT.

- Calculated the RBS strength by using the online tool SalisLab RBS Calculator.

Source

Aspergillus nidulans. The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E coli by Thermo Fisher Scientific.

References

[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385-396.