Coding
D-LDH*

Part:BBa_K2042008

Designed by: Amir Pandi   Group: iGEM16_Evry   (2016-08-30)
Revision as of 18:50, 1 October 2016 by RATOVOMANANA (Talk | contribs)


Engineered D-Lactate dehydrogenase (D-LDH*)

This is the coding sequence of D-lactate deshydrogenase from Lactobacillus delbrueckii 11842. The sequence was condon optimized for Pseudomonas putida and point mutations on three amino acids (D176S, I177R, F178T) were found to efficiently use both NADH and NADPH as cofactors. This enzyme would be useful, as the activity of such an enzyme will not be limited by the availability of NADH or NADPH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/pputida
Parameters
None