Coding
D-LDH*

Part:BBa_K2042008:Design

Designed by: Amir Pandi   Group: iGEM16_Evry   (2016-08-30)
Revision as of 20:21, 1 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)

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Engineered D-Lactate dehydrogenase (D-LDH*)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T) to efficiently use both NADH and NADPH as cofactors. Silent mutation were also introduced for removing restriction site.

Source

Synthetic synthesis

References