Composite

Part:BBa_K2036010

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 07:44, 28 September 2016 by Shareycheng (Talk | contribs)


Cro-TT-pRM-RBS-GFP-LVAssrAtag

It is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example,after constructing PETDurt-1-Cro-TT-pRM-RBS-GFP-LVAssrAtag,IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
HUST-Chian 2016 Build this circuit to test Cro and pRM interaction intensity with contol group:pRM-GFP-LVAssrAtag (BBa_K2036009)

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1036


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Parameters
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