Coding

Part:BBa_K2036004

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 07:11, 28 September 2016 by Shareycheng (Talk | contribs)


ABF2,abscisic acid responsive elements-binding factor 2

Involved in ABA and stress responses and acts as a positive component of glucose signal transduction. Functions as transcriptional activator in the ABA-inducible expression of RD29B. Binds specifically to the ABA-responsive element (ABRE) of the RD29B gene promoter.

We apply ABF2 into our eukaryotic filter .In our system, when signal on is present, snrk2.2 express and phosphorylate ABF2 to activate the promoter RD29A form positive feedback. At the same time, ABF2 triggers the production of the gene of interest.

Fig1:Eukaryote version of Signal Filter

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None