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Part:BBa_K2100001:Experience

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-09-26)
Revision as of 23:08, 17 October 2016 by Sarahcaso (Talk | contribs) (→‎Applications of BBa_K2100001)


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Applications of BBa_K2100001

We characterized the synthetic ERE5 promoter in three cell lines: MCF-7, tHESC, and ISH. All cell lines have endogeneous Estrogen Receptor alpha. We analyzed data from cells induced with estradiol (E2) and uninduced as a control.

Experiment in MCF-7:

We transfected MCF-7 cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

T--MIT--pERE5MCF7.png

Experiment in tHESC:

We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

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