Coding

Part:BBa_K2036003

Designed by: Wangjie Liu   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 07:08, 28 September 2016 by Shareycheng (Talk | contribs)


SnRK2.2,SNF1-related protein kinase 2.2

Together with SRK2I, key component and activator of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as seed germination, Pro accumulation, root growth inhibition, dormancy and seedling growth, and, to a lesser extent, stomatal closure.

We apply SnRK2.2 into our eukaryotic filter. In our system, when signal on is present, SnRK2.2 express and phosphorylate ABF2 to activate the promoter RD29A form positive feedback. At the same time, ABF2 triggers the stable production of target gene.

Fig1:Eukaryote version of Signal Filter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3
    Illegal BamHI site found at 660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None