Composite

Part:BBa_K2036030:Design

Designed by: Zhi Zeng   Group: iGEM16_HUST-China   (2016-09-24)
Revision as of 08:18, 25 September 2016 by WangjieLiu (Talk | contribs) (→‎Source)


PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3756
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BglII site found at 3824
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal BamHI site found at 1816
    Illegal BamHI site found at 2473
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3756
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3756
    Illegal AgeI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5268
    Illegal BsaI.rc site found at 5939


Design Notes

Whole circuit of our designed Signal Filter in eukaryotes

Source

Assemble

References

Regina Antoni, Miguel Gonzalez-Guzman, Lesia Rodriguez, Americo Rodrigues, Gaston A. Pizzio, and Pedro L. Rodriguez Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors. Plant Physiology, February 2012, Vol.158, pp.970–980;

Fen-Fen Soon, Ley-Moy Ng, X. Edward Zhou, Graham M. West, Amanda Kovach, M. H. Eileen Tan, Kelly M. Suino-Powell, Yuanzheng He, Yong Xu, Michael J.Chalmers, Joseph S. Brunzelle, Huiming Zhang, Huaiyu Yang, Hualiang Jiang, Jun Li, Eu-Leong Yong, Sean Cutler, Jian-Kang Zhu, Patrick R. Griffin, Karsten Melcher, and H. Eric Xu Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases. Science. 2012 January 6; 335(6064): 85–88;

Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T, Abe H, Narusaka M, Shinozaki K, Yamaguchi-Shinozaki K Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses. The Plant Journal 2003 Apr; 34(2):137-48.