Reporter

Part:BBa_K1555000:Experience

Designed by: Xu Xuan   Group: iGEM14_NJAU_China   (2014-09-28)
Revision as of 10:01, 24 September 2016 by JosephHung (Talk | contribs) (→‎User Reviews)

This sensor can be activated in low copper concentration and it's truely useful.

2h 0mg.jpg 2h 0mg/L copper ion 2h 1mg.jpg 2h 1mg/L copper ion

Copa.png

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Applications of BBa_K1555000

User Reviews

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HSiTAIWAN

Detecting Copper in Chinese medicine

Objective

     Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine.

Methods

     Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).
     Fluorescence was measured at:

  • Excitation peak: 470nm
  • Emission peak: 508nm

every 30 minutes.

Results

     This part is still specific to copper in Chinese medicine, so we can apply it in Chinese medicine examination.

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Discussion

     Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper.

Review

This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.


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