Regulatory

Part:BBa_J100300:Experience

Designed by: Jose David Hernandez   Group: Campbell M Lab   (2016-09-08)
Revision as of 14:03, 22 September 2016 by YuyiZ (Talk | contribs)


Promoter was ligated during Golden Gate Assembly into E. coli plasmid of strain JM109. Promoter was upstream of RFP (red fluorescent protein) gene. Experiment included positive control with strong known promoter, negative control without a functioning promoter, and two experimental groups with and without 2MC (2-methylcitrate). Data was measured in fluorescence to cell density ration. Data shows that PprpB is non-functioning regardless of presence of 2MC.

PprpB.png

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