Coding

Part:BBa_K1994000

Designed by: Liam Carroll   Group: iGEM16_Warwick   (2016-09-22)
Revision as of 08:59, 22 September 2016 by LCarroll (Talk | contribs)

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dCas9 enzyme

Part containing only dCas9 enzyme with no terminator or promoter. For use in CRISPR/Cas9 systems. Interacts with sgRNA to bind to specific DNA target. Nuclease deficient version of Cas9 enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None