Coding

Part:BBa_K2042002:Design

Designed by: Maria Bargués i Ribera   Group: iGEM16_Evry   (2016-09-02)
Revision as of 17:39, 8 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Source)

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Pseudomonas sp. MBEL 6-19 PHA synthase 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 135
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the DNA coding for the enzyme Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. This sequence was codon optimized for Pseudomonas putida and four amino acid mutations were introduced to increase yields of Polylactic acid (PLA). Silent mutation were also introduced for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the second test for the bioproduction of PLA in Pseudomonas putida pathway.

Source

Pseudomonas sp. MBEL 6-19 gDNA. This sequence was synthetized by IDT

References

Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
http://www.ncbi.nlm.nih.gov/pubmed/19937726

Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.
Yang TH1, Jung YK, Kang HO, Kim TW, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/21221571

Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/23718266