Coding

Part:BBa_K2042001:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-09-02)
Revision as of 17:39, 20 September 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)


Pseudomonas resinovorans PHA synthase 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
    Illegal XhoI site found at 172
    Illegal XhoI site found at 1030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 796


Design Notes

This is the DNA coding for the enzyme Polyhydroxyalkanoate synthase from Pseudomonas resinovorans. This sequence was codon optimized for Pseudomonas putida and four amino acid mutations were introduced to increase yields of PLA. Silent mutation were also introduced for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the second enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.

Source

This sequence was synthetized by IDT


References

Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
http://www.ncbi.nlm.nih.gov/pubmed/19937726

Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.
Yang TH1, Jung YK, Kang HO, Kim TW, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/21221571

Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/23718266