Composite

Part:BBa_K2036025:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 09:15, 3 October 2016 by RichardDeng (Talk | contribs)


Kozak-Snrk2.2-TTADH1-pCyc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 15
    Illegal BamHI site found at 672
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a lactic acid balance circuit in E.coli with lactose signal to initiate. And when the pH environment are within 7 to 9,the host strain will prepare beta-galactosidase for next lactose input.


Source

derived from bacteriaphage lambda operon. And placm, iLDH, patp2 are from the Registry. Beta-galactosidase coding sequence are reffered to pUC119 commerial vector

References

Tim Lynch • B. Joy Erickson • Ruth R. Finkelstein(2012) Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Mol Biol. 2012 Dec;80(6):647-58. doi: 10.1007/s11103-012-9973-3. Epub 2012 Sep 25.
Fen-Fen Soon, Ley-Moy Ng, X. Edward Zhou, Graham M. West, Amanda Kovach, M. H. Eileen Tan, Kelly M. Suino-Powell,Yuanzheng He, Yong Xu, Michael J. Chalmers, Joseph S. Brunzelle, Huiming Zhang（2012）Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases. Science. 2012 Jan 6;335(6064):85-8. doi: 10.1126/science.1215106. Epub 2011 Nov 24.