Intermediate

Part:BBa_K2036007:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 07:55, 19 September 2016 by Shareycheng (Talk | contribs) (→‎Design Notes)

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RBS-CII-RBS-CII, tandem expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One can assemble this part right behind a promoter or at the uptsream of pRE. It can serve as a positive regulation of a certain gene downstream pRE. And according to the Registry: prefix of none coding sequence are supposed to be"GAATTCGCGGCCGCTTCTAGAG", so the scar between two subpart should actually be TACTAGAG but not TACTAG as doucumented in the sequence page.

Source

come from bacteraphage lambda

References