Plasmid_Backbone

Part:BBa_K2077000:Experience

Designed by: Danielle Bauhan, Alexander Lacrampe, Joseph Lee, and Richard Anthony   Group: iGEM16_RHIT   (2016-08-09)
Revision as of 20:51, 9 August 2016 by Xandafish (Talk | contribs) (→‎Applications of BBa_K2077000)


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Applications of BBa_K2077000

T--RHIT--FluorsenceComparisonpicture.PNG

Figure 2. Top Left: By4147 under flourscent light. Top Right By4147 under Normal light. Bottom Left: By 4741 with insert under flourscent light. Bottom Right: By4741 with insert under normal light

We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. By4741 transformed with PBS 416 GPD regained the ability to grow on CSM—URA. Figure 2 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416 GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.

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UNIQ58e15f152c685840-partinfo-00000000-QINU UNIQ58e15f152c685840-partinfo-00000001-QINU