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Coding
Part:BBa_K1983000
Designed by: Vykintas Jauniškis Group: iGEM16_Vilnius-Lithuania (2016-08-01)
Codon optimized AvPAL with C-terminal 6XHis-Tag
This is an improved Stanford-Brown 2013 PAL biobrick part. We have removed the T7 promoter and replaced the N-terminal FLAG-tag with a more common C-terminal 6xHis-tag. The C-terminal tag location was chosen assuming it would less likely affect the stability and folding of the protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1483
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |