Coding
NapDABC

Part:BBa_K2086000:Design

Designed by: Joshua Mueller   Group: iGEM16_UNebraska-Lincoln   (2016-07-27)
Revision as of 01:02, 19 October 2016 by Dannyhdooling (Talk | contribs) (→‎Source)


napDABC (Periplasmic Nitrate Reductase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1991
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 324
    Illegal SapI.rc site found at 238
    Illegal SapI.rc site found at 1734


Design Notes

The part had an illegal restriction site in the NapA gene. The NapDA genes and NapBC genes aren't normally adjacent to each other and so were assembled using HiFi assembly.


Source

The napDABC gene sequence contains parts derived from the genomic DNA of E. coli (MG1655). Certain sequences were changed and ordered from IDT in order to eliminate illegal restriction sites.

References