Project

Part:BBa_K1895000

Designed by: Jake Burton   Group: iGEM16_Newcastle   (2016-07-22)
Revision as of 14:56, 21 September 2016 by Rupert (Talk | contribs)


Electrically induced promoter system v1

This is a composite part comprising the heat shock promoter BBa XXX from MIT 2006, which drives expression of a two-gene operon consisting of a rpoH sigma 32 transcription factor followed by superfolder GFP.

This part was designed for Newcastle iGEM 2016's Culture Shock project in order to induce expression of a downstream gene upon exposure to an electric current. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 738
    Illegal SapI.rc site found at 1087
    Illegal SapI.rc site found at 1335


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