RFC25 "Freiburg standard" parts

Reptorter proteins

Fluorescent proteins

Fluorescent proteins
Protein	Color	BioBrick	Excitation [nm]	Emission [nm]	Quantum Yield
Cerulean		BBa_I757009
GFP	Green	BBa_K648013	484	507	0.60
sfGFP	Green	BBa_K1321337	485	510	0.65
Dronpa (photoactivatable)	Green	BBa_K1680006	503	518
mVenus		BBa_I757008
sYFP2	Yellow	BBa_K1159301	514	527	0.61
eCFP	Cyan	BBa_K1159302	439	476	0.40
mRFP		BBa_K1351021	584	607
mRFP	Red	BBa_K1615089	584	607	0.25

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein	BioBrick	Size [kDa]
Firefly luciferase		61
Renilla luciferase		36
NanoLuc luciferase	BBa_K1159001	19
Gaussia luciferase

Linker domains

Linker
Protein	Sequence	BioBrick	Length [residues]
Short Linker	(Gly-Gly-Ser-Gly)	BBa_K243004	4
Middle Linker	2x (Gly-Gly-Ser-Gly)	BBa_K243005	8
Long Linker	3x (Gly-Gly-Ser-Gly)	BBa_K243006	12

Catalytic domains

Erythromycin Esterase Type II BBa_K1159000

Laccase BBa_K1159002

Laccase TVEL5 BBa_K1615067

TEV Protease BBa_K1159100

Thermonuclease BBa_K1159105

Heroin Esterase BBa_K1615045

CRE recombinase BBa_K1680007

Monoamine Oxidase A BBa_K1615022

Endo-β-Galactosidase BBa_K1483001

α-Galactosidase BBa_K1483002

Acetylgalactosamindase BBa_K1483000

2-oxoglutarate oxygenase/decarboxylase BBa_K1065000

Split proteins

TEV Protease BBa_K1159101 BBa_K1159102

SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201

Binding proteins

Fluorescein binding Anticalin BBa_K1159003

Digoxigenin binding Anticalin BBa_K243003

HER2 binding DARPin BBa_K404302

HER2 binding Affibody BBa_K404302

Nickel Binding Protein (NiBP) BBa_K1321009

Protein tags

Strep-tag II an N-part BBa_K549018

HA tag BBa_K1150016

His₆-tag BBa_I757013

SpyTag BBa_K1159020

Strep-tag II BBa_J18916

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